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Antioxidant pretreatment enhances umbilical cord derived stem cells survival in response to thermal stress in vitro .
- Source :
-
Regenerative medicine [Regen Med] 2020 Mar; Vol. 15 (3), pp. 1441-1453. Date of Electronic Publication: 2020 Apr 27. - Publication Year :
- 2020
-
Abstract
- Aim: Pretreatment of stem cells with antioxidants accelerates their ability to counter oxidative stress and is associated with the overall therapeutic outcome of their transplantation. Material & methods: Wharton Jelly derived mesenchymal stem cells (WJMSCs) were cultured and pretreated with various doses of antioxidants; Vitamin C (Vit C), Vitamin E (Vit E), Vitamin D3 (Vit D3) and their Cocktail, followed by exposure to in vitro heat injury. Assessment of WJMSCs survival, paracrine release, in vitro wound healing and expression of angiogenic and survival markers was conducted. Results: The results displayed an enhanced survival of WJMSCs especially in the case of Cocktail priming. Conclusion: Our data suggest that antioxidant pretreatment of WJMSCs strengthens the endurance of the cells, within stress conditions.
- Subjects :
- Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Humans
In Vitro Techniques
Mesenchymal Stem Cells drug effects
Umbilical Cord drug effects
Wharton Jelly drug effects
Antioxidants pharmacology
Heat-Shock Response
Mesenchymal Stem Cells cytology
Umbilical Cord cytology
Wharton Jelly cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1746-076X
- Volume :
- 15
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Regenerative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 32339058
- Full Text :
- https://doi.org/10.2217/rme-2019-0090