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Spatiotemporal Analysis Reveals Overlap of Key Proepicardial Markers in the Developing Murine Heart.
- Source :
-
Stem cell reports [Stem Cell Reports] 2020 May 12; Vol. 14 (5), pp. 770-787. Date of Electronic Publication: 2020 Apr 30. - Publication Year :
- 2020
-
Abstract
- The embryonic epicardium, originating from the proepicardial organ (PEO), provides a source of multipotent progenitors for cardiac lineages, including pericytes, fibroblasts, and vascular smooth muscle cells. Maximizing the regenerative capacity of the adult epicardium depends on recapitulating embryonic cell fates. The potential of the epicardium to contribute coronary endothelium is unclear, due to conflicting Cre-based lineage trace data. Controversy also surrounds when epicardial cell fate becomes restricted. Here, we systematically investigate expression of five widely used epicardial markers, Wt1, Tcf21, Tbx18, Sema3d, and Scx, over the course of development. We show overlap of markers in all PEO and epicardial cells until E13.5, and find no evidence for discrete proepicardial sub-compartments that might contribute coronary endothelium via the epicardial layer. Our findings clarify a number of prevailing discrepancies and support the notion that epicardium-derived cell fate, to form fibroblasts or mural cells, is specified after epithelial-mesenchymal transition, not pre-determined within the PEO.<br /> (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors genetics
Basic Helix-Loop-Helix Transcription Factors metabolism
Fibroblasts cytology
Fibroblasts metabolism
Mice
Mice, Inbred C57BL
Mouse Embryonic Stem Cells cytology
Muscle Cells cytology
Muscle Cells metabolism
Pericardium embryology
Pericardium metabolism
Pericytes cytology
Pericytes metabolism
Semaphorins genetics
Semaphorins metabolism
T-Box Domain Proteins genetics
T-Box Domain Proteins metabolism
WT1 Proteins genetics
WT1 Proteins metabolism
Cell Lineage
Mouse Embryonic Stem Cells metabolism
Pericardium cytology
Subjects
Details
- Language :
- English
- ISSN :
- 2213-6711
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Stem cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 32359445
- Full Text :
- https://doi.org/10.1016/j.stemcr.2020.04.002