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A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2142, pp. 181-195. - Publication Year :
- 2020
-
Abstract
- Pigs are highly relevant to model human in utero Zika virus (ZIKV) infection because both species have similar physiology, genetics, immunity, fetal brain development, and postnatal brain growth. The virus causes persistent in utero infection and replicates in the fetal brain, fetal membranes, and placenta. Subclinical persistent in utero infection in mid-gestation also increases interferon alpha (IFN-α) levels in fetal blood plasma and amniotic fluid. Moreover, we demonstrated altered IFN-α responses in porcine offspring affected with subclinical in utero ZIKV infection. Elevated levels of in utero type I interferons were suggested to play a role in fetal pathology. Thus, the porcine model may provide an understanding of ZIKV-induced immunopathology in fetuses and sequelae in offspring, which is important for the development of targeted interventions. Here, we describe surgery, ultrasound-guided in utero injection, postoperative monitoring, sampling, and cytokine testing protocols.
- Subjects :
- Amniotic Fluid metabolism
Animals
Blood Chemical Analysis methods
Blood Chemical Analysis veterinary
Female
Fetal Blood metabolism
Fetoscopy methods
Fetoscopy veterinary
Injections
Interferon-alpha analysis
Interferon-alpha blood
Pregnancy
Ultrasonography, Interventional methods
Ultrasonography, Interventional veterinary
Ultrasonography, Prenatal methods
Ultrasonography, Prenatal veterinary
Zika Virus physiology
Disease Models, Animal
Fetal Diseases diagnostic imaging
Fetal Diseases metabolism
Fetal Diseases pathology
Fetal Diseases virology
Interferon-alpha metabolism
Pregnancy Complications, Infectious diagnostic imaging
Pregnancy Complications, Infectious metabolism
Pregnancy Complications, Infectious pathology
Swine
Zika Virus Infection diagnostic imaging
Zika Virus Infection metabolism
Zika Virus Infection pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2142
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32367368
- Full Text :
- https://doi.org/10.1007/978-1-0716-0581-3_15