Supplemented powdered coconut water (ACP-406 ® ) promotes growth of goat secondary follicles and oocyte meiotic resumption.

The objective of this study was to test the efficiency of powdered coconut water (ACP-406 ^® ) base-medium without or with the addition of supplements on in vitro culture of isolated goat secondary follicles. Follicles were cultured for 18 days in α-MEM or in ACP-406 ^® , both without supplements (referred to as α-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as α-MEM ⁺ and ACP ⁺ ). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after in vitro maturation were evaluated. At the end of culture, ACP-406 ^® base-medium (without or with supplements) showed a higher (P < 0.05) percentage of normal follicles than α-MEM (without or with supplements). Antrum formation was similar among α-MEM ⁺ , ACP and ACP ⁺ , and significantly higher than α-MEM without supplements. The follicular diameter was greater in ACP ⁺ than α-MEM, and similar to other treatments. Moreover, fully and daily grown rates were higher (P < 0.05) in ACP-406 ^® base-medium (without or with supplements) than α-MEM (without or with supplements). Levels of GSH were similar between ACP ⁺ and α-MEM ⁺ treatments. Both ACP ⁺ and α-MEM ⁺ allowed meiotic resumption without a significant difference between the two groups. In conclusion, supplemented ACP-406 ^® base-medium maintained follicular survival and promoted the development as well as meiotic resumption of isolated goat secondary follicles cultured in vitro for 18 days.
Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
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