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Capnocytophaga felis sp. nov. isolated from the feline oral cavity.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 May; Vol. 70 (5), pp. 3355-3360. - Publication Year :
- 2020
-
Abstract
- Four strains, KC07070 <superscript>T</superscript> , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO <subscript>2</subscript> and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070 <superscript>T</superscript> had identity to those of the type strains of Capnocytophaga canimorsus (97.7 %), Capnocytophaga cynodegmi (97.8 %) and Capnocytophaga canis (97.4 %) and 91.2-93.8% identity to those of other species of the genus Capnocytophaga . The major cellular fatty acids of KC07070 <superscript>T</superscript> were iso-C <subscript>15 : 0</subscript> (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070 <superscript>T</superscript> was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070 <superscript>T</superscript> had digital DNA-DNA hybridization (dDDH) values of 26.2-27.6% and average nucleotide identity (ANI) values of 75.4-83.3 % to the type strains of the closest relatives, C. canimorsus , C. cynodegmi and C. canis . These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070 <superscript>T</superscript> represents a novel species, for which we propose the name Capnocytophaga felis sp. nov., with type strain KC07070 <superscript>T</superscript> (=JCM 32681 <superscript>T</superscript> =DSM 107251 <superscript>T</superscript> ).
- Subjects :
- Animals
Bacterial Typing Techniques
Base Composition
Capnocytophaga isolation & purification
DNA, Bacterial genetics
Fatty Acids chemistry
Japan
Nucleic Acid Hybridization
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Capnocytophaga classification
Cats microbiology
Mouth microbiology
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 70
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32375938
- Full Text :
- https://doi.org/10.1099/ijsem.0.004176