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Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease.
- Source :
-
Journal of the American Heart Association [J Am Heart Assoc] 2020 May 18; Vol. 9 (10), pp. e015929. Date of Electronic Publication: 2020 May 09. - Publication Year :
- 2020
-
Abstract
- Background Peripheral artery disease (PAD) is a manifestation of atherosclerosis characterized by reduced blood flow to the lower extremities and mobility loss. Preliminary evidence suggests PAD damages skeletal muscle, resulting in muscle impairments that contribute to functional decline. We sought to determine whether PAD is associated with an altered macrophage profile in gastrocnemius muscles and whether muscle macrophage populations are associated with impaired muscle phenotype and walking performance in patients with PAD. Methods and Results Macrophages, satellite cells, and extracellular matrix in gastrocnemius muscles from 25 patients with PAD and 7 patients without PAD were quantified using immunohistochemistry. Among patients with PAD, both the absolute number and percentage of cluster of differentiation (CD) 11b+CD206+ M2-like macrophages positively correlated to satellite cell number ( r =0.461 [ P =0.023] and r =0.416 [ P =0.042], respectively) but not capillary density or extracellular matrix. The number of CD11b+CD206- macrophages negatively correlated to 4-meter walk tests at normal ( r =-0.447, P =0.036) and fast pace ( r =-0.510, P =0.014). Extracellular matrix occupied more muscle area in PAD compared with non-PAD (8.72±2.19% versus 5.30±1.03%, P <0.001) and positively correlated with capillary density ( r =0.656, P <0.001). Conclusions Among people with PAD, higher CD206+ M2-like macrophage abundance was associated with greater satellite cell numbers and muscle fiber size. Lower CD206- macrophage abundance was associated with better walking performance. Further study is needed to determine whether CD206+ macrophages are associated with ongoing reparative processes enabling skeletal muscle adaptation to damage with PAD. Registration URL: https://www.clinicaltrials.gov; Unique identifiers: NCT00693940, NCT01408901, NCT0224660.
- Subjects :
- Adaptation, Physiological
Aged
Biomarkers analysis
CD11b Antigen analysis
Case-Control Studies
Cross-Sectional Studies
Extracellular Matrix pathology
Female
Humans
Macrophages immunology
Male
Membrane Glycoproteins analysis
Microvascular Density
Middle Aged
Muscle, Skeletal physiopathology
Observational Studies as Topic
Peripheral Arterial Disease diagnosis
Peripheral Arterial Disease physiopathology
Phenotype
Randomized Controlled Trials as Topic
Receptors, Immunologic analysis
Satellite Cells, Skeletal Muscle pathology
Macrophages pathology
Muscle, Skeletal pathology
Peripheral Arterial Disease pathology
Walking
Subjects
Details
- Language :
- English
- ISSN :
- 2047-9980
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of the American Heart Association
- Publication Type :
- Academic Journal
- Accession number :
- 32390569
- Full Text :
- https://doi.org/10.1161/JAHA.118.015929