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Human CD31 on Swine Endothelial Cells Induces SHP-1 Phosphorylation in Macrophages.
- Source :
-
Transplantation proceedings [Transplant Proc] 2020 Jul - Aug; Vol. 52 (6), pp. 1913-1915. Date of Electronic Publication: 2020 May 10. - Publication Year :
- 2020
-
Abstract
- Background: Innate immunity by natural killer (NK) cells, macrophages, and neutrophils cause severe rejections in xenotransplantation. Therefore, the development of strategies for suppressing macrophages has considerable potential in practical applications of xenotransplantation. Recently, we found that human CD31 on swine endothelial cells (SECs) suppresses neutrophil-mediated xenogeneic rejection through homophilic binding. Since a significant amount of CD31 is expressed not only on neutrophils but also on macrophages, we studied the function of human CD31 in macrophage-mediated cytotoxicity.<br />Methods: SECs and hCD31-transfected SECs (SEC/hCD31) were co-cultured with macrophages and cytotoxicity by macrophages was evaluated with water-soluble tetrazolium salt, or WST-8, assay. To confirm whether or not inhibitory signals are induced by hCD31 homophilic binding, the phosphorylation of the enzyme SHP-1 was investigated with Western blotting.<br />Results: No suppression of cytotoxicity was induced in macrophages that had been co-cultured with SEC/CD31. However, phosphorylation of SHP-1 was induced in macrophages that had been co-cultured with SEC/hCD31.<br />Conclusions: Human CD31 on SEC may induce not only inhibitory signals but also activation signals via the binding to other receptors for hCD31.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Coculture Techniques
Cytotoxicity, Immunologic immunology
Graft Rejection immunology
Humans
Phosphorylation
Platelet Endothelial Cell Adhesion Molecule-1 genetics
Swine
Transfection
Endothelial Cells immunology
Heterografts immunology
Macrophages metabolism
Platelet Endothelial Cell Adhesion Molecule-1 immunology
Protein Tyrosine Phosphatase, Non-Receptor Type 6 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2623
- Volume :
- 52
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Transplantation proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 32402461
- Full Text :
- https://doi.org/10.1016/j.transproceed.2020.01.140