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B cell depletion in murine lupus using cytotoxic T lymphocytes in vivo: Feasibility and benefit.
- Source :
-
Cellular immunology [Cell Immunol] 2020 Jul; Vol. 353, pp. 104117. Date of Electronic Publication: 2020 May 05. - Publication Year :
- 2020
-
Abstract
- Given the promising results in human lupus with B cell depletion, we tested whether in vivo cytotoxic T lymphocyte (CTL) could eliminate autoreactive B cells in the setting of murine lupus. Using the parent-into-F1 (P → F1) model to generate CTL that eliminate B cells, we found that transfer ofNZB parental splenocytes into lupus-prone female NZB/W F1 mice resulted in profound B cell reduction whereas NZW → F1 mice exhibited defective B cell elimination. Using pre-disease or early disease B/W mice as hosts, NZB → F1 mice exhibited B cell depletion and improved proteinuria but no improvement in survival whereas NZW → F1 mice had significantly reduced proteinuria and prolonged survival. Thus, despite the defective IL-2 environment in B/W F1 mice, generation of CTL and B cell depletion is feasible in NZB → F1 mice. The surprising increase in survival for NZW → F1 mice despite defective B cell elimination suggests that NZW splenocytes may contain a beneficial down regulatory cell.<br /> (Published by Elsevier Inc.)
- Subjects :
- Animals
Autoantibodies immunology
Autoimmunity immunology
Disease Models, Animal
Feasibility Studies
Female
Lupus Erythematosus, Systemic immunology
Male
Mice
Mice, Inbred NZB
T-Lymphocytes, Cytotoxic metabolism
B-Lymphocytes immunology
Lupus Erythematosus, Systemic therapy
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2163
- Volume :
- 353
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 32408197
- Full Text :
- https://doi.org/10.1016/j.cellimm.2020.104117