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Expression of CA2 and CA9 carbonic anhydrases in ulcerative colitis and ulcerative colitis-associated colorectal cancer.
- Source :
-
Pathology international [Pathol Int] 2020 Aug; Vol. 70 (8), pp. 523-532. Date of Electronic Publication: 2020 May 14. - Publication Year :
- 2020
-
Abstract
- Ulcerative colitis (UC) is characterized by chronic inflammation in the colonic mucosa and submucosa with repeating relapse and remission, but the pathogenesis is unknown. Patients with long-standing UC are at high risk of neoplasm development. The aim of the present study was to identify molecules whose expression is associated with UC and UC-associated colorectal cancer (UCCA). Biopsy specimens from UC and normal colonic mucosae were analyzed using a proteomics approach, in which carbonic anhydrase 2 (CA2) was identified as a molecule downregulated in UC mucosae. Immunohistochemically, CA2 expression was detected in normal and diverticulitis mucosal epithelia, and its expression decreased as UC activity increased. CA2 expression was almost undetectable in UCCA. We also analyzed the expression of another carbonic anhydrase, CA9, and its upstream molecule, hypoxia-inducible factor-1α (HIF-1α), both of which are induced under hypoxic conditions. It was revealed that CA9 expression was relatively low in normal, diverticulitis and UC mucosae, and was upregulated in UCCA. HIF-1α expression was consistently low in all tissue types examined. In conclusion, these results suggest that CA2 and CA9 may be possible indicators of UC activity and UCCA development, respectively.<br /> (© 2020 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.)
- Subjects :
- Antigens, Neoplasm metabolism
Biomarkers, Tumor metabolism
Blotting, Western methods
Carbonic Anhydrase II metabolism
Carbonic Anhydrase IX metabolism
Colorectal Neoplasms etiology
Humans
Immunohistochemistry methods
Intestinal Mucosa metabolism
Intestinal Mucosa pathology
Carbonic Anhydrases metabolism
Colitis, Ulcerative complications
Colitis-Associated Neoplasms diagnosis
Colitis-Associated Neoplasms etiology
Colorectal Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1440-1827
- Volume :
- 70
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Pathology international
- Publication Type :
- Academic Journal
- Accession number :
- 32410301
- Full Text :
- https://doi.org/10.1111/pin.12949