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The Effect of the Food Matrix on the In Vitro Bio-Accessibility and IgE Reactivity of Peanut Allergens.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2020 Jul; Vol. 64 (14), pp. e1901093. Date of Electronic Publication: 2020 Jun 29. - Publication Year :
- 2020
-
Abstract
- Scope: Factors such as food processing, the food matrix, and antacid medication may affect the bio-accessibility of proteins in the gastrointestinal tract and hence their allergenic activity. However, at present they are poorly understood.<br />Methods and Results: Roasted peanut flour was incorporated into either a chocolate dessert or cookie matrix and bio-accessibility were assessed using an in vitro digestion system comprising a model chew and simulated gastric and duodenal digestion. Protein digestion was monitored by SDS-PAGE and immunoreactivity analyzed by immunoblotting and immunoassay. IgE reactivity was assessed by immunoassay using serum panels from peanut-allergic subjects. Roasted peanut flour proteins proved highly digestible following gastro-duodenal digestion even when incurred into a food matrix, with only low molecular weight polypeptides of Mr < 8 kDa remaining. When gastric digestion was performed at pH 6.5 (simulating the effect of antacid medication), peanut proteins are not digested; subsequent duodenal digestion is also limited. IgE reactivity of the major peanut allergens Ara h 1, Ara h 2, and Ara h 6, although reduced, was retained after oral-gastro-duodenal digestion irrespective of digestion conditions employed.<br />Conclusion: Peanut allergen bio-accessibility is unaffected by the dessert or cookie matrices whilst high intra-gastric pH conditions render allergens more resistant to digestion.<br /> (© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- 2S Albumins, Plant immunology
2S Albumins, Plant pharmacokinetics
Antigens, Plant immunology
Antigens, Plant pharmacology
Arachis immunology
Biological Availability
Digestion
Food Handling methods
Humans
Hydrogen-Ion Concentration
Membrane Proteins pharmacokinetics
Plant Proteins immunology
Arachis chemistry
Immunoglobulin E immunology
Peanut Hypersensitivity immunology
Plant Proteins pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 64
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 32420668
- Full Text :
- https://doi.org/10.1002/mnfr.201901093