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In silico identification of MAPK14-related lncRNAs and assessment of their expression in breast cancer samples.
- Source :
-
Scientific reports [Sci Rep] 2020 May 20; Vol. 10 (1), pp. 8316. Date of Electronic Publication: 2020 May 20. - Publication Year :
- 2020
-
Abstract
- Mitogen-activated protein kinase (MAP kinase) pathways participate in regulation of several cellular processes involved in breast carcinogenesis. A number of non-coding RNAs including both microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) regulate or being regulated by MAPKs. We performed an in-silico method for identification of MAPKs with high number of interactions with miRNAs and lncRNAs. Bioinformatics approaches revealed that MAPK14 ranked first among MAPKs. Subsequently, we identified miRNAs and lncRNAs that were predicted to be associated with MAPK14. Finally, we selected four lncRNAs with higher predicted scores (NORAD, HCG11, ZNRD1ASP and TTN-AS1) and assessed their expression in 80 breast cancer tissues and their adjacent non-cancerous tissues (ANCTs). Expressions of HCG11 and ZNRD1ASP were lower in tumoral tissues compared with ANCTs (P values < 0.0001). However, expression levels of MAPK14 and NORAD were not significantly different between breast cancer tissues and ANCTs. A significant association was detected between expression of HCG11 and estrogen receptor (ER) status in a way that tumors with up-regulation of this lncRNA were mostly ER negative (P value = 0.04). Expressions of ZNRD1ASP and HCG11 were associated with menopause age and breast feeding duration respectively (P values = 0.02 and 0.04 respectively). There was a trend towards association between ZNRD1ASP expression and patients' age of cancer diagnosis. Finally, we detected a trend toward association between expression of NORAD and history of hormone replacement therapy (P value = 0.06). Expression of MAPK14 was significantly higher in grade 1 tumors compared with grade 2 tumors (P value = 0.02). Consequently, the current study provides evidences for association between lncRNA expressions and reproductive factors or tumor features.
- Subjects :
- Adult
Breast Neoplasms enzymology
Breast Neoplasms genetics
Computer Simulation
Female
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
MAP Kinase Signaling System
Mammary Glands, Human metabolism
MicroRNAs metabolism
Middle Aged
Mitogen-Activated Protein Kinase 14 genetics
Breast Neoplasms metabolism
Mitogen-Activated Protein Kinase 14 metabolism
RNA, Long Noncoding metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32433496
- Full Text :
- https://doi.org/10.1038/s41598-020-65421-2