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Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase ( PdVIK ) Under Abiotic Stresses.
- Source :
-
Genes [Genes (Basel)] 2020 May 19; Vol. 11 (5). Date of Electronic Publication: 2020 May 19. - Publication Year :
- 2020
-
Abstract
- The date palm ( Khalas ) is an extremophile plant that can adapt to various abiotic stresses including drought and salinity. Salinity tolerance is a complex trait controlled by numerous genes. Identification and functional characterization of salt-responsive genes from the date palm is fundamental to understand salinity tolerance at the molecular level in this plant species. In this study, a salt-inducible vascular highway 1-interacting kinase ( PdVIK ) that is a MAP kinase kinase kinase (MAPKKK) gene from the date palm, was functionally characterized using in vitro and in vivo strategies. PdVIK , one of the 597 kinases encoded by the date palm genome possesses an ankyrin repeat domain and a kinase domain. The recombinant PdVIK protein exhibited phosphotyrosine activity against myelin basic protein (MBP) substrate. Overexpression of PdVIK in yeast significantly improved its tolerance to salinity, LiCl, and oxidative stresses. Transgenic Arabidopsis seedlings overexpressing PdVIK displayed improved tolerance to salinity, osmotic, and oxidative stresses as assessed by root growth assay. The transgenic lines grown in the soil also displayed modulated salt response, compared to wild-type controls as evaluated by the overall plant growth and proline levels. Likewise, the transgenic lines exhibited drought tolerance by maintaining better relative water content (RWC) compared to non-transgenic control plants. Collectively, these results implicate the involvement of PdVIK in modulating the abiotic stress response of the date palm.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Droughts
Extremophiles genetics
Extremophiles growth & development
Gene Expression Regulation, Plant genetics
MAP Kinase Kinase Kinases genetics
Myelin Basic Protein genetics
Oxidative Stress genetics
Phoeniceae growth & development
Plants, Genetically Modified genetics
Plants, Genetically Modified growth & development
Salinity
Salt Tolerance genetics
Seedlings genetics
Seedlings growth & development
Sodium Chloride adverse effects
Adaptation, Physiological genetics
Phoeniceae genetics
Protein Kinases genetics
Stress, Physiological genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 11
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 32438658
- Full Text :
- https://doi.org/10.3390/genes11050568