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A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Jul 12; Vol. 528 (1), pp. 99-104. Date of Electronic Publication: 2020 May 24. - Publication Year :
- 2020
-
Abstract
- A novel Kunitz-type neurotoxin peptide that inhibited voltage-gated sodium channel was purified and characterized from the skin secretions of rufous-spotted torrent frog, Amolops loloensis. It has a 240-bp cDNA encoding an 79-amino acid residue (aa) precursor protein containing 6 half-cysteines. The precursor was proven to release a 57-aa mature peptide with amino acid sequence, DRNPICNLPPKEGFCLWMMRRSFFNPSKGRCDTFGYRGCGGNKNNFETPRACKEACG. The mature was named amotoxin. Amotoxin shares sequence homology with other Kunitz-type toxins and also has three cysteine bridges. Amotoxin showed an inhibitory ability against trypsin with an inhibitory constant (Ki) of 0.087 μM. To the best of our knowledge, this is the first gene-encoded neurotoxin found in Amolops loloensis. Recombinant amotoxin showed similar functional properties as the native amotoxin. The functional properties of amotoxin may provide insights into the ecological adaptation of amphibians and deepen our understanding about the biological function spectrum of amphibian skin peptides.<br />Competing Interests: Declaration of competing interest The authors claim no conflicts of interest.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
DNA, Complementary genetics
Female
Ion Channels metabolism
Male
Neurotoxins chemistry
Neurotoxins genetics
Peptides chemistry
Peptides genetics
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Neurotoxins isolation & purification
Peptides isolation & purification
Ranidae metabolism
Skin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 528
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 32460958
- Full Text :
- https://doi.org/10.1016/j.bbrc.2020.05.051