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Characterisation of a subpopulation of CD133 + cancer stem cells from Chinese patients with oral squamous cell carcinoma.
- Source :
-
Scientific reports [Sci Rep] 2020 Jun 01; Vol. 10 (1), pp. 8875. Date of Electronic Publication: 2020 Jun 01. - Publication Year :
- 2020
-
Abstract
- Cancer stem cells (CSCs) play a critical role in cancer development and growth. The aim of this study was to identify and isolate CSCs from populations of primary oral squamous cell carcinoma (OSCC) cells, which were obtained from OSCC specimens and identified by cell morphology and immunohistochemical staining for keratin. CD133 <superscript>+</superscript> cells detected by flow cytometry comprised 0.41 ± 0.06% of primary OSCC cells and were isolated from primary OSCC cell populations using magnetic-activated cell sorting, revealing that 93.39% of high-purity CD133 <superscript>+</superscript> cells were in the G0/G1 phase of the cell cycle. Additionally, the growth rate of CD133 <superscript>+</superscript> cells was higher than that of CD133 <superscript>-</superscript> cells, and in vivo tumourigenesis experiments showed that the tumourigenic ability of CD133 <superscript>+</superscript> cells was markedly stronger than that of CD133 <superscript>-</superscript> cells. Moreover, CD133 <superscript>+</superscript> cells showed increased chemotherapeutic resistance to cisplatin and higher self-renewal ability according to sphere-formation assay, as well as higher mRNA levels of stemness-associated genes, including NANOG, SOX2, ALDH1A1, and OCT4. These results indicated that OSCC cells, which share certain characteristics of CSCs, harbour CD133 <superscript>+</superscript> cells potentially responsible for OSCC aggressiveness, suggesting CD133 as a potential prognostic marker and therapeutic target.
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Antineoplastic Agents therapeutic use
Asian People
Carcinoma, Squamous Cell diagnosis
Carcinoma, Squamous Cell drug therapy
Cell Proliferation drug effects
Cell Self Renewal
China
Cisplatin pharmacology
Cisplatin therapeutic use
Drug Resistance, Neoplasm
G1 Phase
Humans
Mice
Mice, Inbred BALB C
Mouth Neoplasms diagnosis
Mouth Neoplasms drug therapy
Nanog Homeobox Protein genetics
Nanog Homeobox Protein metabolism
Neoplastic Stem Cells cytology
Octamer Transcription Factor-3 genetics
Octamer Transcription Factor-3 metabolism
RNA, Messenger metabolism
Transplantation, Heterologous
AC133 Antigen metabolism
Carcinoma, Squamous Cell pathology
Mouth Neoplasms pathology
Neoplastic Stem Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32483269
- Full Text :
- https://doi.org/10.1038/s41598-020-64947-9