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Oxytocin Controls Chondrogenesis and Correlates with Osteoarthritis.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 May 31; Vol. 21 (11). Date of Electronic Publication: 2020 May 31. - Publication Year :
- 2020
-
Abstract
- This study investigated the relationship of oxytocin (OT) to chondrogenesis and osteoarthritis (OA). Human bone marrow and multipotent adipose-derived stem cells were cultured in vitro in the absence or presence of OT and assayed for mRNA transcript expression along with histological and immunohistochemical analyses. To study the effects of OT in OA in vivo, a rat model and a human cohort of 63 men and 19 women with hand OA and healthy controls, respectively, were used. The baseline circulating OT, interleukin-6, leptin, and oestradiol levels were measured, and hand X-ray examinations were performed for each subject. OT induced increased aggrecan , collagen ( Col ) X , and cartilage oligomeric matrix protein mRNA transcript levels in vitro, and the immunolabelling experiments revealed a normalization of Sox9 and Col II protein expression levels. No histological differences in lesion severity were observed between rat OA groups. In the clinical study, a multivariate analysis adjusted for age, body mass index, and leptin levels revealed a significant association between OA and lower levels of OT (odds ratio = 0.77; p = 0.012). Serum OT levels are reduced in patients with hand OA, and OT showed a stimulatory effect on chondrogenesis. Thus, OT may contribute to the pathophysiology of OA.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Aged
Animals
Body Mass Index
Bone Marrow metabolism
Cell Culture Techniques
Chondrocytes metabolism
Collagen Type II blood
Estradiol blood
Extracellular Matrix metabolism
Female
Humans
Immunohistochemistry
Interleukin-1beta metabolism
Interleukin-6 blood
Leptin blood
Male
Middle Aged
Multivariate Analysis
Osteoarthritis metabolism
Oxytocin blood
RNA, Messenger metabolism
Rats
SOX9 Transcription Factor blood
SOX9 Transcription Factor metabolism
Stem Cells cytology
Chondrogenesis drug effects
Osteoarthritis drug therapy
Oxytocin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 32486506
- Full Text :
- https://doi.org/10.3390/ijms21113966