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A sensitive "Switch-on" phosphorescent probe for ferrous iron quantification in drug and In vitro imaging of living cells.
- Source :
-
Talanta [Talanta] 2020 Sep 01; Vol. 217, pp. 121097. Date of Electronic Publication: 2020 Apr 30. - Publication Year :
- 2020
-
Abstract
- Iron plays an important role in various physiological processes. However, the detailed biological functions of iron have not been sufficiently explored because of a lack of effective methods to monitoring iron, especially the labile ferrous ion (Fe <superscript>2+</superscript> ). In the current study, a novel turn-on phosphorescent probe for Fe <superscript>2+</superscript> quantification and visualization has been proposed based on the hybrid nanocomposite of manganese dioxide and gemini iridium complex (MnO <subscript>2</subscript> -GM-Ir). The surfactant-like GM-Ir with positive charges was beneficial to combine with the negatively charged manganese dioxide (MnO <subscript>2</subscript> ) nanosheets, and thus endowing the MnO <subscript>2</subscript> -GM-Ir nanocomposite excellent dispersion ability in the water as well as efficiently avoiding the interference to the detection caused by the agglomeration of nanocomposite. Phosphorescence of GM-Ir was effectively quenched by MnO <subscript>2</subscript> nanosheets through fluorescence resonance energy transfer (FRET) and the inner filter effect (IFE), while the phosphorescence could be significantly recovered in the presence of Fe <superscript>2+</superscript> via a selective Fe <superscript>2+</superscript> -mediated reduction of MnO <subscript>2</subscript> nanosheets, indicating a highly-specific selectivity towards Fe <superscript>2+</superscript> with a low detection limit (80 nM). The drug test assay and in vitro imaging studies further proved that the MnO <subscript>2</subscript> -GM-Ir nanocomposite could be employed as a promising probe for the quantitative detection of exogenous Fe <superscript>2+</superscript> in drug and in vitro imaging of living cells.<br />Competing Interests: Declaration of competing interest There are no conflicts to declare.<br /> (Copyright © 2020. Published by Elsevier B.V.)
- Subjects :
- Animals
Calcitriol analogs & derivatives
Calcitriol chemistry
Cell Line, Tumor
Fluorescence Resonance Energy Transfer
Ions analysis
Iridium chemistry
Manganese Compounds chemical synthesis
Manganese Compounds chemistry
Mice
Nanocomposites chemistry
Oxides chemical synthesis
Oxides chemistry
Particle Size
Spectrometry, Fluorescence
Surface Properties
Ferrous Compounds analysis
Fluorescent Dyes chemistry
Optical Imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 217
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 32498867
- Full Text :
- https://doi.org/10.1016/j.talanta.2020.121097