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Dual RNA-seq provides novel insight into the roles of dksA from Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers ( Larimichthys crocea ).
- Source :
-
Zoological research [Zool Res] 2020 Jul 18; Vol. 41 (4), pp. 410-422. - Publication Year :
- 2020
-
Abstract
- Pseudomonas plecoglossicida is a rod-shaped, gram-negative bacterium with flagella. It causes visceral white spot disease and high mortality in Larimichthys crocea during culture, resulting in serious economic loss. Analysis of transcriptome and quantitative real-time polymerase chain reaction (PCR) data showed that dksA gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides (log <subscript>2</subscript> FC=3.12, P <0.001). RNAi of five shRNAs significantly reduced the expression of dksA in P. plecoglossicida , and the optimal silencing efficiency was 96.23%. Compared with wild-type strains, the symptoms of visceral white spot disease in L. crocea infected with RNAi strains were reduced, with time of death delayed by 48 h and mortality reduced by 25%. The dksA silencing led to a substantial down-regulation in cellular component-, flagellum-, and ribosome assembly-related genes in P. plecoglossicida , and the significant up-regulation of fliC may be a way in which virulence is maintained in P. plecoglossicida . The GO and KEGG results showed that RNAi strain infection in L. crocea led to the down-regulation of inflammatory factor genes in immune-related pathways, which were associated with multiple immune response processes. Results also showed that dksA was a virulence gene in P. plecoglossicida . Compared with the wild-type strains, RNAi strain infection induced a weaker immune response in L. crocea .
- Subjects :
- Animals
Bacterial Proteins metabolism
Fish Diseases microbiology
Gene Expression Regulation
Host-Pathogen Interactions genetics
Pseudomonas Infections immunology
Pseudomonas Infections microbiology
RNA, Bacterial analysis
RNA-Seq veterinary
Virulence Factors metabolism
Bacterial Proteins genetics
Fish Diseases immunology
Perciformes
Pseudomonas physiology
Pseudomonas Infections veterinary
Virulence Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2095-8137
- Volume :
- 41
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zoological research
- Publication Type :
- Academic Journal
- Accession number :
- 32521576
- Full Text :
- https://doi.org/10.24272/j.issn.2095-8137.2020.048