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Clinical relevance and functional significance of cell-free microRNA-1260b expression profiles in infiltrative myxofibrosarcoma.
- Source :
-
Scientific reports [Sci Rep] 2020 Jun 10; Vol. 10 (1), pp. 9414. Date of Electronic Publication: 2020 Jun 10. - Publication Year :
- 2020
-
Abstract
- Infiltrative tumor growth into adjacent soft tissues is a major cause of the frequent recurrence and tumor-related death of myxofibrosarcoma (MFS), but no useful biomarkers reflecting tumor burden and infiltrative growth are available. While emerging evidence suggests a diagnostic and functional role of extracellular/circulating microRNA (miRNA) in various malignant diseases, their significance in MFS patients remains unknown. Global miRNA profiling identified four upregulated miRNAs in MFS patient sera and culture media of MFS cells. Among these, serum miR-1260b level was significantly upregulated in patient serum discriminating from healthy individuals and closely correlated with clinical status and tumor dynamics in MFS-bearing mice. In addition, high miR-1260b expression in serum was correlated with radiological tail-like patterns, characteristic of the infiltrative MFS. The extracellular miR-1260b was embedded in tumor-derived extracellular vesicles (EVs) and promoted cellular invasion of MFS through the downregulation of PCDH9 in the adjacent normal fibroblasts. Collectively, circulating miR-1260b expression may represent a novel diagnostic target for tumor monitoring of this highly aggressive sarcoma. Moreover, EV-miR-1260b could act as a transfer messenger to adjacent cells and mediate the infiltrative growth of MFS, providing new insights into the mechanism of infiltrative nature via crosstalk between tumor cells and their microenvironment.
- Subjects :
- Aged
Aged, 80 and over
Animals
Biomarkers, Tumor genetics
Cell Line, Tumor
Down-Regulation genetics
Extracellular Vesicles genetics
Female
Fibroblasts pathology
Gene Expression Profiling methods
Histiocytoma, Malignant Fibrous genetics
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Recurrence, Local genetics
Soft Tissue Neoplasms genetics
Tumor Microenvironment genetics
Up-Regulation genetics
Circulating MicroRNA genetics
Fibrosarcoma genetics
MicroRNAs genetics
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32523124
- Full Text :
- https://doi.org/10.1038/s41598-020-66120-8