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Characterization of the IncHI 2 plasmid pTW4 harboring tet(M) from an isolate of Escherichia coli ST162.
- Source :
-
The Journal of antibiotics [J Antibiot (Tokyo)] 2020 Dec; Vol. 73 (12), pp. 876-880. Date of Electronic Publication: 2020 Jun 12. - Publication Year :
- 2020
-
Abstract
- To investigate the genetic features and biological costs of the plasmid pTW4 harboring tet(M) in an isolate of Escherichia coli ST162 from a duck. The complete nucleotide sequence of plasmid pTW4 was determined. The characteristics of plasmid pTW4 in E. coli were investigated by stability and direct competition assays. pTW4 is an IncHI <subscript>2</subscript> -type plasmid that contained the resistant genes tet(M), floR, strAB, sul2, rmtB, and bla <subscript>CMY-2</subscript> . Tet(M) is located in the composite transposon Tn6539 within the multidrug resistant (MDR) region on this plasmid. Furthermore, the resistance gene rmtB and bla <subscript>CMY-2</subscript> were found outside the MDR region. The plasmid pTW4 remained stable in the host strain E. coli J53 after passage under an antibiotic-free environment for 7 days. However, the strain E. coli J53/pTW4 showed a fitness disadvantage of 6% per ten generations in the process of growth competition with E. coli J53. In conclusion, the plasmid pTW4, a mobile MDR vehicle, may promote the dissemination of tet(M), floR, rmtB, strAB, sul2, and bla <subscript>CMY-2</subscript> among bacteria and then, but it appears to confer growth disadvantage to the host.
Details
- Language :
- English
- ISSN :
- 1881-1469
- Volume :
- 73
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of antibiotics
- Publication Type :
- Academic Journal
- Accession number :
- 32528162
- Full Text :
- https://doi.org/10.1038/s41429-020-0337-y