A new steroidal glycoside from the fruits of Solanum myriacanthum .

A new cholestane-type steroidal glycoside, solamyriaside A ( 1 ), was isolated from the fruits of Solanum myriacanthum Dunal (Solanaceae), along with two known steroidal glycosides, namely, solaviaside A ( 2 ) and aculeatiside A ( 3 ), and three known steroidal alkaloid glycosides, namely, solamargine ( 4 ), khasianine ( 5 ) and solasonine ( 6 ), which were isolated for the first time from this plant. Based on spectroscopic data as well as chemical evidence, 1 was determined to be 3- O -α-L-rhamnopyranosyl-(1→2)- O -[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosyl-22 R ,25 R -cholest-5-ene-3β,16α,22,26-tetraol 26- O -β-D-glucopyranoside. The cytotoxic activity of 1 - 6 against HL-60 human promyelocytic leukaemia cells was examined. Compounds 4 - 6 showed cytotoxic activity. Among them, 4 exhibited the strongest activity with an IC ₅₀ value of 4.64 ± 0.17 μM, similar to the activity of cisplatin, a positive control.