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Bat SARS-Like WIV1 coronavirus uses the ACE2 of multiple animal species as receptor and evades IFITM3 restriction via TMPRSS2 activation of membrane fusion.
- Source :
-
Emerging microbes & infections [Emerg Microbes Infect] 2020 Dec; Vol. 9 (1), pp. 1567-1579. - Publication Year :
- 2020
-
Abstract
- Diverse SARS-like coronaviruses (SL-CoVs) have been identified from bats and other animal species. Like SARS-CoV, some bat SL-CoVs, such as WIV1, also use angiotensin converting enzyme 2 (ACE2) from human and bat as entry receptor. However, whether these viruses can also use the ACE2 of other animal species as their receptor remains to be determined. We report herein that WIV1 has a broader tropism to ACE2 orthologs than SARS-CoV isolate Tor2. Among the 9 ACE2 orthologs examined, human ACE2 exhibited the highest efficiency to mediate the infection of WIV1 pseudotyped virus. Our findings thus imply that WIV1 has the potential to infect a wide range of wild animals and may directly jump to humans. We also showed that cell entry of WIV1 could be restricted by interferon-induced transmembrane proteins (IFITMs). However, WIV1 could exploit the airway protease TMPRSS2 to partially evade the IFITM3 restriction. Interestingly, we also found that amphotericin B could enhance the infectious entry of SARS-CoVs and SL-CoVs by evading IFITM3-mediated restriction. Collectively, our findings further underscore the risk of exposure to animal SL-CoVs and highlight the vulnerability of patients who take amphotericin B to infection by SL-CoVs, including the most recently emerging (SARS-CoV-2).
- Subjects :
- Angiotensin-Converting Enzyme 2
Animals
Betacoronavirus classification
HEK293 Cells
Humans
Rats
Receptors, Coronavirus
Severe acute respiratory syndrome-related coronavirus physiology
Viverridae
Betacoronavirus physiology
Chiroptera virology
Membrane Proteins metabolism
Peptidyl-Dipeptidase A metabolism
RNA-Binding Proteins metabolism
Receptors, Virus metabolism
Serine Endopeptidases metabolism
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 2222-1751
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Emerging microbes & infections
- Publication Type :
- Academic Journal
- Accession number :
- 32602823
- Full Text :
- https://doi.org/10.1080/22221751.2020.1787797