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Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host.
- Source :
-
Molecular and biochemical parasitology [Mol Biochem Parasitol] 2020 Jul; Vol. 238, pp. 111296. Date of Electronic Publication: 2020 Jun 27. - Publication Year :
- 2020
-
Abstract
- Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.<br />Competing Interests: Declaration of Competing Interest None.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Chromatography, Liquid
Extracellular Vesicles metabolism
Extracellular Vesicles parasitology
Lipidomics
Mesocricetus blood
Principal Component Analysis
Proteome metabolism
Protozoan Proteins blood
Schistosoma mansoni pathogenicity
Schistosomiasis mansoni blood
Tandem Mass Spectrometry
Blood Proteins metabolism
Extracellular Vesicles chemistry
Host-Parasite Interactions
Mesocricetus parasitology
Phospholipids blood
Schistosoma mansoni metabolism
Schistosomiasis mansoni parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9428
- Volume :
- 238
- Database :
- MEDLINE
- Journal :
- Molecular and biochemical parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 32603736
- Full Text :
- https://doi.org/10.1016/j.molbiopara.2020.111296