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Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice.
- Source :
-
Molecular imaging [Mol Imaging] 2020 Jan-Dec; Vol. 19, pp. 1536012120934965. - Publication Year :
- 2020
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Abstract
- Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.<br />Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.<br />Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg <superscript>-1</superscript> and 0.3 mg kg <superscript>-1</superscript> doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.<br />Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.
Details
- Language :
- English
- ISSN :
- 1536-0121
- Volume :
- 19
- Database :
- MEDLINE
- Journal :
- Molecular imaging
- Publication Type :
- Academic Journal
- Accession number :
- 32609570
- Full Text :
- https://doi.org/10.1177/1536012120934965