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Fe 3 O 4 @CuS-based immunochromatographic test strips and their application to label-free and dual-readout detection of Escherichia coli O157:H7 in food.
- Source :
-
Food chemistry [Food Chem] 2020 Dec 01; Vol. 332, pp. 127398. Date of Electronic Publication: 2020 Jun 24. - Publication Year :
- 2020
-
Abstract
- Herein, a label-free and dual-readout immunochromatographic test strip (ITS) for the sensitive detection of Escherichia coli (E. coli) O157:H7 by taking advantages of the strong capture ability of Fe <subscript>3</subscript> O <subscript>4</subscript> @CuS nanostructures (NSs) towards bacteria and their ultrahigh photothermal effects (PTEs) was reported. Especially, without the customarily antibody (Ab)-labeled probe, Fe <subscript>3</subscript> O <subscript>4</subscript> @CuS NSs could be adsorbed onto the surfaces of bacteria to form Fe <subscript>3</subscript> O <subscript>4</subscript> @CuS-bacteria conjugates and then trapped by immobilized Abs on the test line (T-line), forming a characteristic yellow band. After direct immunoreactions, the PTEs of Fe <subscript>3</subscript> O <subscript>4</subscript> @CuS NSs endowed T-line to be irradiated by an 808 nm infrared light, obtaining satisfactory sensitivity and accuracy. Under optimal conditions, E. coli O157:H7, as low as 10 <superscript>3</superscript> and 10 <superscript>2</superscript> CFU/mL, could be monitored in colorimetric and photothermal modes. Additionally, E. coli O157:H7 was successfully detected in beef, chicken, milk and honey samples by this proposed platform with a recovery of 80-120%.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Cattle
Chickens microbiology
Chromatography, Affinity instrumentation
Escherichia coli O157 immunology
Food Microbiology instrumentation
Honey microbiology
Limit of Detection
Milk microbiology
Reagent Strips chemistry
Red Meat microbiology
Chromatography, Affinity methods
Copper chemistry
Escherichia coli O157 isolation & purification
Ferric Compounds chemistry
Food Microbiology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 332
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32610260
- Full Text :
- https://doi.org/10.1016/j.foodchem.2020.127398