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Labeling of DNA Probes by Nick Translation.
- Source :
-
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2020 Jul 01; Vol. 2020 (7), pp. 100602. Date of Electronic Publication: 2020 Jul 01. - Publication Year :
- 2020
-
Abstract
- In this method, E. coli DNA Pol I binds to a nick or short gap in duplex DNA. The 5' → 3' exonuclease activity of Pol I then removes nucleotides from one strand of the DNA, creating a template for the synthesis of DNA by the 5' → 3' polymerase activity of Pol I. The simultaneous elimination of nucleotides from the 5' side and the addition of nucleotides to the 3' side result in movement of the nick (nick translation) along the DNA, which becomes labeled to high specific activity.<br /> (© 2020 Cold Spring Harbor Laboratory Press.)
- Subjects :
- DNA Probes metabolism
DNA, Bacterial metabolism
Deoxyribonuclease I metabolism
Deoxyribonucleotides genetics
Deoxyribonucleotides metabolism
Escherichia coli metabolism
DNA Breaks, Single-Stranded
DNA Probes genetics
DNA, Bacterial genetics
Escherichia coli genetics
In Situ Nick-End Labeling methods
Subjects
Details
- Language :
- English
- ISSN :
- 1559-6095
- Volume :
- 2020
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cold Spring Harbor protocols
- Publication Type :
- Academic Journal
- Accession number :
- 32611783
- Full Text :
- https://doi.org/10.1101/pdb.prot100602