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Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency.
- Source :
-
Scientific reports [Sci Rep] 2020 Jul 01; Vol. 10 (1), pp. 10796. Date of Electronic Publication: 2020 Jul 01. - Publication Year :
- 2020
-
Abstract
- Germ cell depletion in recipient testes is indispensable for successful transplantation of spermatogonial stem cells. However, we found that such treatment had an adverse effect on spermatogenesis of orthotopically transplanted donor testis tissues. In the donor tissue, the frequency of stimulated by retinoic acid (RA) 8 (STRA8) expression was reduced in germ cells, suggesting that RA signalling indispensable for spermatogenesis was attenuated in germ cell-depleted recipient testes. In this context, germ cell depletion diminished expression of testicular Aldh1a2, which is responsible for testicular RA synthesis, while Cyp26b1, which is responsible for testicular RA metabolism, was still expressed even after germ cell depletion, suggesting an alteration of the RA synthesis/metabolism ratio. These observations suggested that RA insufficiency was one of the causes of the defective donor spermatogenesis. Indeed, repetitive RA administrations significantly improved donor spermatogenesis to produce fertile offspring without any side effects. These findings may contribute to improving fertility preservation techniques for males, especially to prevent iatrogenic infertility induced by chemotherapy in prepubertal cancer patients.
- Subjects :
- Aldehyde Dehydrogenase 1 Family biosynthesis
Animals
Gene Expression Regulation, Enzymologic
Humans
Male
Mice
Retinal Dehydrogenase biosynthesis
Retinoic Acid 4-Hydroxylase biosynthesis
Organ Transplantation
Spermatogenesis
Spermatogonia enzymology
Testis enzymology
Testis transplantation
Tretinoin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32612133
- Full Text :
- https://doi.org/10.1038/s41598-020-67595-1