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Lack of influence of cyclosporine on antigen presentation to lysozyme-specific T cell hybridomas.
- Source :
-
Transplantation [Transplantation] 1988 Aug; Vol. 46 (2 Suppl), pp. 44S-48S. - Publication Year :
- 1988
-
Abstract
- Cyclosporine (CsA) was tested for its ability to inhibit antigen presentation by spleen cells or the B lymphoma line A20 to T cell hybridomas specific for hen egg-white lysozyme (HEL). Antigen-presenting cells (APC) were treated with CsA or nonimmunosuppressive derivatives thereof during or prior to encounter with antigen. The APC were then washed extensively and incubated in CsA-free medium for 6 hr before the T hybridoma cells were added. Under these conditions, CsA had no effect on antigen presentation up to the cytostatic regimen (1 microgram/ml). Omission of the 6-hr interval between CsA treatment of APC and the addition of T hybridoma resulted in inhibition of interleukin 2 production, although the CsA concentrations required were 10-75-fold higher than the ones inhibiting T cells directly (IC50: 100-150 ng/ml vs. 2-10 ng/ml). The responses to both HEL and a synthetic peptide of HEL sequence 105-120 were inhibited, indicating that the step influenced by the drug was not antigen-processing. The nonimmunosuppressive derivatives remained ineffective under these conditions. The results illustrate that the carryover of CsA from APC to T cells can mimic a drug effect on antigen presentation. Therefore, the demonstration of a CsA effect on antigen presentation can only be considered as conclusive when the readout of APC function is not a T cell response.
- Subjects :
- Animals
Antigen-Presenting Cells immunology
Cell Division drug effects
Hybridomas
In Vitro Techniques
Interleukin-2 biosynthesis
Lymphocyte Activation drug effects
Mice
Muramidase
Peptide Fragments immunology
Antigen-Presenting Cells drug effects
Cyclosporins pharmacology
Lymphocyte Cooperation drug effects
T-Lymphocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0041-1337
- Volume :
- 46
- Issue :
- 2 Suppl
- Database :
- MEDLINE
- Journal :
- Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 3261467
- Full Text :
- https://doi.org/10.1097/00007890-198808001-00009