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The actin nucleator Cobl organises the terminal web of enterocytes.
- Source :
-
Scientific reports [Sci Rep] 2020 Jul 07; Vol. 10 (1), pp. 11156. Date of Electronic Publication: 2020 Jul 07. - Publication Year :
- 2020
-
Abstract
- Brush borders of intestinal epithelial cells are mandatory for nutrient uptake. Yet, which actin nucleators are crucial for forming the F-actin bundles supporting microvilli and the actin filaments of the terminal web, in which microvilli are rooted, is unknown. We show that mice lacking the actin nucleator Cobl surprisingly did not display reduced microvilli densities or changes in microvillar F-actin bundles or microvilli diameter but particularly in the duodenum displayed increased microvillar length. Interestingly, Cobl-deficient mice furthermore showed a significant widening of the terminal web. Quantitative analyses of high-resolution cryo-scanning electron microscopy (EM) of deep-etched duodenum samples revealed that Cobl is specifically important for the formation of fine filaments in the central terminal web that connect the apical structure of the terminal web underlying the plasma membrane, the microvilli rootlets and the basal structure of the terminal web with each other. Thus, the actin nucleator Cobl is critically involved in generating one of the cellular structures of the brush border-decorated apical cortex of enterocytes representing the absorptive intestinal surface.
- Subjects :
- Actins metabolism
Animals
Blotting, Western
Cell Membrane metabolism
Cell Membrane ultrastructure
Cryoelectron Microscopy methods
Enterocytes ultrastructure
Intestinal Mucosa metabolism
Intestinal Mucosa ultrastructure
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Electron, Scanning methods
Microvilli physiology
Microvilli ultrastructure
Real-Time Polymerase Chain Reaction
Enterocytes metabolism
Microfilament Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32636403
- Full Text :
- https://doi.org/10.1038/s41598-020-66111-9