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Monoclonal antibodies against leucoagglutinin-reactive human T-lymphocyte surface components. II. Studies on the mechanism of K46M-induced activation and determination of the frequency of responding cells.
- Source :
-
Scandinavian journal of immunology [Scand J Immunol] 1988 Dec; Vol. 28 (6), pp. 773-82. - Publication Year :
- 1988
-
Abstract
- A monoclonal antibody, K46M (IgM kappa), obtained after immunization with leucoagglutinin (La)-reactive T-cell surface components, stimulated human lymphocytes to proliferate. It induced maximal proliferation at greater than 20 micrograms IgM/ml after 3-4 days of culture. Cells stimulated by K46M produced interleukin 2 (IL-2) and gamma interferon (IFN-gamma) and expressed receptors for IL-2 and transferrin. The majority of the activated cells were phenotypically T cells as defined by monoclonal antibodies against CD3 and CD2, and an increase in the K46M-positive cells was also observed during the activation period. K46M-activated cells display major histocompatibility complex (MHC)-unrestricted cytotoxicity against several cultured target cells. The frequencies of the cytotoxic and of the proliferative precursor cells were determined using a limiting dilution assay. K46M seems to activate a larger fraction of cytotoxic precursor cells against Molt 4 than against K562, but the statistical significance of these observations requires further exploration. Both K46M or La activated 40% of PBL to proliferate, whereas 70% of PBL were induced by OKT3. However, the frequency of K46M-activated cells was 40% only when the lymphocytes were plated at low cell densities, i.e. less than 0.5 cells per well. At higher densities an inhibition of proliferation was seen that resulted in a biphasic response curve, indicating that the activation of PBL by K46M was not a single hit event. This was not found with either La or OKT3. Whether K46M, in contrast to OKT3 and La, activates a subpopulation with suppressor activity remains to be established.
- Subjects :
- Animals
Cytotoxicity Tests, Immunologic
Fabaceae
Humans
Mice
Mitogens physiology
Plant Lectins
Plants, Medicinal
Stem Cells immunology
T-Lymphocytes, Cytotoxic immunology
Antibodies, Monoclonal physiology
Antigens, Surface immunology
Leukocyte Count
Lymphocyte Activation
Phytohemagglutinins immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0300-9475
- Volume :
- 28
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 3266027
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1988.tb01511.x