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Two-omics data revealed commonalities and differences between Rpv12- and Rpv3-mediated resistance in grapevine.
Two-omics data revealed commonalities and differences between Rpv12- and Rpv3-mediated resistance in grapevine.
- Source :
-
Scientific reports [Sci Rep] 2020 Jul 22; Vol. 10 (1), pp. 12193. Date of Electronic Publication: 2020 Jul 22. - Publication Year :
- 2020
-
Abstract
- Plasmopara viticola is the causal agent of grapevine downy mildew (DM). DM resistant varieties deploy effector-triggered immunity (ETI) to inhibit pathogen growth, which is activated by major resistance loci, the most common of which are Rpv3 and Rpv12. We previously showed that a quick metabolome response lies behind the ETI conferred by Rpv3 TIR-NB-LRR genes. Here we used a grape variety operating Rpv12-mediated ETI, which is conferred by an independent locus containing CC-NB-LRR genes, to investigate the defence response using GC/MS, UPLC, UHPLC and RNA-Seq analyses. Eighty-eight metabolites showed significantly different concentration and 432 genes showed differential expression between inoculated resistant leaves and controls. Most metabolite changes in sugars, fatty acids and phenols were similar in timing and direction to those observed in Rpv3-mediated ETI but some of them were stronger or more persistent. Activators, elicitors and signal transducers for the formation of reactive oxygen species were early observed in samples undergoing Rpv12-mediated ETI and were paralleled and followed by the upregulation of genes belonging to ontology categories associated with salicylic acid signalling, signal transduction, WRKY transcription factors and synthesis of PR-1, PR-2, PR-5 pathogenesis-related proteins.
- Subjects :
- Databases, Genetic
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Plant
Metabolome
Peronospora isolation & purification
Plant Diseases genetics
Plant Diseases microbiology
Plant Leaves metabolism
Plant Leaves microbiology
Plant Proteins genetics
Principal Component Analysis
RNA, Plant chemistry
RNA, Plant genetics
RNA, Plant metabolism
RNA-Seq
Vitis microbiology
Disease Resistance genetics
Genomics methods
Plant Proteins metabolism
Vitis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32699241
- Full Text :
- https://doi.org/10.1038/s41598-020-69051-6