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Alpha-momorcharin enhances Nicotiana benthamiana resistance to tobacco mosaic virus infection through modulation of reactive oxygen species.
- Source :
-
Molecular plant pathology [Mol Plant Pathol] 2020 Sep; Vol. 21 (9), pp. 1212-1226. Date of Electronic Publication: 2020 Jul 26. - Publication Year :
- 2020
-
Abstract
- Alpha-momorcharin (α-MMC), a member of the plant ribosomal inactivating proteins (RIPs) family, has been proven to exhibit important biological properties in animals, including antiviral, antimicrobial, and antitumour activities. However, the mechanism by which α-MMC increases plant resistance to viral infections remains unclear. To study the effect of α-MMC on plant viral defence and how α-MMC increases plant resistance to viruses, recombinant DNA and transgenic technologies were employed to investigate the role of α-MMC in Nicotiana benthamiana resistance to tobacco mosaic virus (TMV) infection. Treatment with α-MMC produced through DNA recombinant technology or overexpression of α-MMC mediated by transgenic technology alleviated TMV-induced oxidative damage and reduced the accumulation of reactive oxygen species (ROS) during TMV-green fluorescent protein infection of N. benthamiana. There was a significant decrease in TMV replication in the upper leaves following local α-MMC treatment and in α-MMC-overexpressing plants relative to control plants. These results suggest that application or overexpression of α-MMC in N. benthamiana increases resistance to TMV infection. Finally, our results showed that overexpression of α-MMC up-regulated the expression of ROS scavenging-related genes. α-MMC confers resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme-encoding genes. Overall, our study revealed a new crosstalk mechanism between α-MMC and ROS during resistance to viral infection and provides a framework to understand the molecular mechanisms of α-MMC in plant defence against viral pathogens.<br /> (© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
- Subjects :
- Gene Expression
Plant Diseases immunology
Plant Diseases virology
Plants, Genetically Modified
Ribosome Inactivating Proteins genetics
Nicotiana genetics
Nicotiana virology
Disease Resistance
Plant Diseases prevention & control
Reactive Oxygen Species metabolism
Ribosome Inactivating Proteins metabolism
Nicotiana immunology
Tobacco Mosaic Virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1364-3703
- Volume :
- 21
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular plant pathology
- Publication Type :
- Academic Journal
- Accession number :
- 32713165
- Full Text :
- https://doi.org/10.1111/mpp.12974