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Maslinic acid prevents IL-1β-induced inflammatory response in osteoarthritis via PI3K/AKT/NF-κB pathways.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2021 Mar; Vol. 236 (3), pp. 1939-1949. Date of Electronic Publication: 2020 Jul 30. - Publication Year :
- 2021
-
Abstract
- Osteoarthritis (OA) is a degenerative joint disease characterized by destruction of articular cartilage. The inflammatory response is the most important factor affecting the disease process. As interleukin-1β (IL-1β) stimulates several key mediators in the inflammatory response, it plays a major role in the pathogenesis of OA. Maslinic acid (MA) is a natural compound distributed in olive fruit. Previous studies have found that maslinic acid has an inhibitory effect on inflammation, but its specific role in the progression of OA disease has not been studied so far. In this study, we aim to assess the protective effect of MA on OA progression by in vitro and in vivo experiments. Our results indicate that, in IL-1β-induced inflammatory response, MA is effective in attenuating some major inflammatory mediators such as nitric oxide (NO) and prostaglandin E2, and inhibits the expression of IL-6, inducible nitric oxide synthase, cyclooxygenase-2, and tumor necrosis factor-α (TNF-α) in a concentration-dependent manner. Also, MA downregulated the expression levels of thrombospondin motif 5 (ADAMTS5) and matrix metalloproteinase 13 in chondrocytes, resulting in reduced degradation of its extracellular matrix. Mechanistically, MA exhibits an anti-inflammatory effect by inactivating the PI3K/AKT/NF-κB pathway. In vivo, the protective effect of MA on OA development can be detected in a surgically induced mouse OA model. In summary, these findings suggest that MA can be used as a safe and effective potential OA therapeutic strategy.<br /> (© 2020 Wiley Periodicals LLC.)
- Subjects :
- Aged
Animals
Cell Survival drug effects
Chondrocytes drug effects
Chondrocytes metabolism
Chondrocytes pathology
Cyclooxygenase 2 metabolism
Dinoprostone metabolism
Disease Models, Animal
Extracellular Matrix drug effects
Extracellular Matrix metabolism
Female
Humans
Inflammation complications
Interleukin-1beta adverse effects
Interleukin-6 metabolism
Male
Mice, Inbred C57BL
Models, Biological
NF-KappaB Inhibitor alpha metabolism
Nitric Oxide metabolism
Nitric Oxide Synthase Type II metabolism
Protein Transport drug effects
Proteolysis drug effects
Transcription Factor RelA metabolism
Triterpenes chemistry
Triterpenes pharmacology
Tumor Necrosis Factor-alpha metabolism
Mice
Inflammation prevention & control
NF-kappa B metabolism
Osteoarthritis drug therapy
Osteoarthritis metabolism
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction
Triterpenes therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 236
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 32730652
- Full Text :
- https://doi.org/10.1002/jcp.29977