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A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.
- Source :
-
Cell host & microbe [Cell Host Microbe] 2020 Sep 09; Vol. 28 (3), pp. 486-496.e6. Date of Electronic Publication: 2020 Jul 03. - Publication Year :
- 2020
-
Abstract
- There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.<br />Competing Interests: Declaration of Interests K.C. is a member of the scientific advisory board of Integrum Scientific, LLC.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Angiotensin-Converting Enzyme 2
Animals
Antiviral Agents pharmacology
Betacoronavirus genetics
Betacoronavirus physiology
COVID-19
COVID-19 Vaccines
Cell Line
Chlorocebus aethiops
Coronavirus Infections drug therapy
Coronavirus Infections genetics
Coronavirus Infections immunology
Coronavirus Infections prevention & control
Coronavirus Infections therapy
Drug Evaluation, Preclinical
Host Microbial Interactions drug effects
Host Microbial Interactions genetics
Host Microbial Interactions physiology
Humans
Mutation
Neutralization Tests
Pandemics prevention & control
Peptidyl-Dipeptidase A genetics
Peptidyl-Dipeptidase A physiology
Pneumonia, Viral prevention & control
Pneumonia, Viral therapy
Receptors, Virus genetics
Receptors, Virus physiology
Recombination, Genetic
SARS-CoV-2
Serine Endopeptidases physiology
Spike Glycoprotein, Coronavirus genetics
Vero Cells
Vesicular stomatitis Indiana virus genetics
Viral Vaccines genetics
Viral Vaccines immunology
Virus Internalization
Virus Replication genetics
COVID-19 Drug Treatment
Betacoronavirus pathogenicity
Coronavirus Infections virology
Pneumonia, Viral virology
Spike Glycoprotein, Coronavirus physiology
Vesicular stomatitis Indiana virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1934-6069
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell host & microbe
- Publication Type :
- Academic Journal
- Accession number :
- 32738193
- Full Text :
- https://doi.org/10.1016/j.chom.2020.06.020