Antibiofilm and immunological properties of lectin purified from shrimp Penaeus semisulcatus.

Authors :: Preetham E
Lakshmi S
Wongpanya R
Vaseeharan B
Arockiaraj J
Olsen RE
Source :: Fish & shellfish immunology [Fish Shellfish Immunol] 2020 Nov; Vol. 106, pp. 776-782. Date of Electronic Publication: 2020 Jul 31.
Publication Year :: 2020
Abstract: Penaeid prawns are considered as most demanding fishery resources. The current study aims to purify and characterize lectin from the haemolymph of Penaeus semisulcatus. The semisulcatus-lectin was purified by affinity chromatography using mannose coupled Sepharose CL-4B column and purified lectin exhibited a single band of 66 kDa in SDS-PAGE. The purity and crystalline structure of purified lectin was confirmed by HPLC and X-ray diffraction analysis. Semisulcatus-lectin exhibited yeast agglutination activity against Saccharomyces cerevisiae and agglutinated human erythrocytes. Semisulcatus-lectin was evaluated for phenol oxidase activation and phagocytic activities. It was observed that semisulcatus-lectin had antibacterial activity against Gram-negative Vibrio parahaemolyticus and Aeromonas hydrophila, suggesting a potential therapeutic strategy in aquaculture industry for disease management.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Subjects :: Aeromonas hydrophila drug effects
Aeromonas hydrophila physiology
Agglutination drug effects
Animals
Anti-Bacterial Agents isolation & purification
Arthropod Proteins isolation & purification
Biofilms drug effects
Erythrocytes drug effects
Hemolymph chemistry
Humans
Lectins isolation & purification
Monophenol Monooxygenase metabolism
Saccharomyces cerevisiae drug effects
Vibrio parahaemolyticus drug effects
Vibrio parahaemolyticus physiology
Anti-Bacterial Agents pharmacology
Arthropod Proteins pharmacology
Lectins pharmacology
Penaeidae

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