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Synergistic Effect of a Pleuromutilin Derivative with Tetracycline against Streptococcus suis In Vitro and in the Neutropenic Thigh Infection Model.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2020 Aug 01; Vol. 25 (15). Date of Electronic Publication: 2020 Aug 01. - Publication Year :
- 2020
-
Abstract
- Tetracycline (TET) has been widely used in the treatment of Streptococcus suis ( S. suis ) infection. However, it was found that the efficacy of many antibiotics in S. suis decreased significantly, especially tetracycline. In this study, GML-12 (a novel pleuromutilin derivative) was used in combination with TET against 12 S. suis isolates. In the checkerboard assay, the TET/GML-12 combination exhibited synergistic and additive effects against S. suis isolates ( n = 12). In vitro time-killing assays and in vivo therapeutic experiments were used to confirm the synergistic effect of the TET/GML-12 combination against S. suis strains screened based on an FICI ≤ 0.5. In time-killing assays, the TET/GML-12 combination showed a synergistic effect or an additive effect against three isolates with a bacterial reduction of over 2.4-log <subscript>10</subscript> CFU/mL compared with the most active monotherapy. Additionally, the TET/GML-12 combination displayed potent antimicrobial activity against four isolates in a mouse thigh infection model. These results suggest that the TET/GML-12 combination may be a potential therapeutic strategy for S. suis infection.
- Subjects :
- Animals
Anti-Bacterial Agents toxicity
Bacterial Zoonoses drug therapy
Bacterial Zoonoses microbiology
Disease Models, Animal
Diterpenes toxicity
Drug Synergism
Drug Therapy, Combination
Female
Humans
In Vitro Techniques
Mice
Mice, Inbred ICR
Microbial Sensitivity Tests
Neutropenia drug therapy
Neutropenia microbiology
Polycyclic Compounds toxicity
Streptococcal Infections microbiology
Streptococcus suis isolation & purification
Swine
Swine Diseases drug therapy
Swine Diseases microbiology
Pleuromutilins
Anti-Bacterial Agents administration & dosage
Diterpenes administration & dosage
Polycyclic Compounds administration & dosage
Streptococcal Infections drug therapy
Streptococcus suis drug effects
Tetracycline administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 25
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 32752180
- Full Text :
- https://doi.org/10.3390/molecules25153522