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Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
- Source :
-
Current medical science [Curr Med Sci] 2020 Aug; Vol. 40 (4), pp. 614-617. Date of Electronic Publication: 2020 Aug 29. - Publication Year :
- 2020
-
Abstract
- The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.
- Subjects :
- Adult
Aged
COVID-19
COVID-19 Testing
China epidemiology
Coronavirus Infections epidemiology
Humans
Infection Control methods
Medical Laboratory Personnel
Middle Aged
Molecular Diagnostic Techniques methods
Occupational Exposure prevention & control
Pandemics
Pneumonia, Viral epidemiology
RNA, Viral analysis
RNA, Viral genetics
SARS-CoV-2
Temperature
Time Factors
Betacoronavirus genetics
Betacoronavirus isolation & purification
Clinical Laboratory Techniques methods
Coronavirus Infections diagnosis
Coronavirus Infections virology
Pneumonia, Viral diagnosis
Pneumonia, Viral virology
Real-Time Polymerase Chain Reaction methods
Virus Inactivation
Subjects
Details
- Language :
- English
- ISSN :
- 2523-899X
- Volume :
- 40
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Current medical science
- Publication Type :
- Academic Journal
- Accession number :
- 32767258
- Full Text :
- https://doi.org/10.1007/s11596-020-2224-y