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How to use the development of individual Drosophila larvae as a metabolic sensor.
- Source :
-
Journal of insect physiology [J Insect Physiol] 2020 Oct; Vol. 126, pp. 104095. Date of Electronic Publication: 2020 Aug 09. - Publication Year :
- 2020
-
Abstract
- Metabolic research is a challenge because of the variety of data within experimental series and the difficulty of replicating results among scientific groups. The fruit fly, Drosophila melanogaster, is a cost-effective and reliable pioneer model to screen dietary variables for metabolic research. One of the main reasons for problems in this field are differences in food recipes, diet-associated microbial environments and the pharmacokinetic behavior of nutrients across the gut-blood barrier. To prevent such experimental shortcomings, a common strategy is to pool scores of subjects into one sample to create an average statement. However, this approach lacks information about the biological spread and may provoke misleading interpretations. We propose to use the developmental rate of individual Drosophila larvae as a metabolic sensor. To do so, we introduce here a 96-well plate-based assay, which allows screening for multiple variables including food quality, microbial load, and genetic differences. We demonstrate that on a diet that is rich in calories, pupation is sensitive to the variation of dietary lipid compounds and that genotypes considered as wild-types/controls produce different developmental profiles. Our platform is suited for later automation and represents a potent high-throughput screening tool for the pharmacology and food industry. If used systematically, our assay could become a powerful reference tool to compare the quality of used dietary configurations with published benchmark recipes.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animal Feed microbiology
Animals
Cholesterol analysis
Food Industry
Larva growth & development
Larva metabolism
Lipid Metabolism
Lipids isolation & purification
Models, Animal
Biological Assay methods
Diet
Drosophila melanogaster growth & development
Drosophila melanogaster metabolism
Nutrients metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1611
- Volume :
- 126
- Database :
- MEDLINE
- Journal :
- Journal of insect physiology
- Publication Type :
- Academic Journal
- Accession number :
- 32783958
- Full Text :
- https://doi.org/10.1016/j.jinsphys.2020.104095