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Anthocyanins Isolated from Vitis coignetiae Pulliat Enhances Cisplatin Sensitivity in MCF-7 Human Breast Cancer Cells through Inhibition of Akt and NF-κB Activation.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2020 Aug 09; Vol. 25 (16). Date of Electronic Publication: 2020 Aug 09. - Publication Year :
- 2020
-
Abstract
- Anthocyanins isolated from Vitis coignetiae Pulliat (Meoru in Korea) (AIMs) have various anti-cancer properties by inhibiting Akt and NF-κB which are involved in drug resistance. Cisplatin (CDDP) is one of the popular anti-cancer agents. Studies reported that MCF-7 human breast cancer cells have high resistance to CDDP compared to other breast cancer cell lines. In this study, we confirmed CDDP resistance of MCF-7 cells and tested whether AIMs can overcome CDDP resistance of MCF-7 cells. Cell viability assay revealed that MCF-7 cells were more resistant to CDDP treatment than MDA-MB-231 breast cancer cells exhibiting aggressive and high cancer stem cell phenotype. AIMs significantly augmented the efficacy of CDDP with synergistic effects on MCF-7 cells. Molecularly, Western blot analysis revealed that CDDP strongly increased Akt and moderately reduced p-NF-κB and p-IκB and that AIMs inhibited CDDP-induced Akt activation, and augmented CDDP-induced reduction of p-NF-κB and p-IκB in MCF-7 cells. In addition, AIMs significantly downregulated an anti-apoptotic protein, XIAP, and augmented PARP-1 cleavage in CDDP-treated MCF-7 cells. Moreover, under TNF-α treatment, AIMs augmented CDDP efficacy with inhibition of NF-κB activation on MCF-7 cells. In conclusion, AIMs enhanced CDDP sensitivity by inhibiting Akt and NF-κB activity of MCF-7 cells that show relative intrinsic CDDP resistance.
- Subjects :
- Antineoplastic Agents pharmacology
Apoptosis
Breast Neoplasms metabolism
Breast Neoplasms pathology
Cell Cycle
Cell Movement
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Humans
NF-kappa B genetics
Proto-Oncogene Proteins c-akt genetics
Tumor Cells, Cultured
Anthocyanins pharmacology
Breast Neoplasms drug therapy
Cisplatin pharmacology
Drug Resistance, Neoplasm
NF-kappa B metabolism
Plant Extracts pharmacology
Proto-Oncogene Proteins c-akt metabolism
Vitis chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 25
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 32784919
- Full Text :
- https://doi.org/10.3390/molecules25163623