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Imaging Hg 2+ -Induced Oxidative Stress by NIR Molecular Probe with "Dual-Key-and-Lock" Strategy.
- Source :
-
Analytical chemistry [Anal Chem] 2020 Sep 01; Vol. 92 (17), pp. 12002-12009. Date of Electronic Publication: 2020 Aug 14. - Publication Year :
- 2020
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Abstract
- Mercury (Hg) is considered an extremely toxic heavy metal which is extremely harmful to both the human body and environment. In addition, Hg <superscript>2+</superscript> -induced oxidative stress also exerts a crucial role to play in pathophysiological mechanisms of mercury toxicity. Thus, efficient and specific fluorescent probes for imaging Hg <superscript>2+</superscript> -induced oxidative stress are necessary. In the present study, we rationally design a novel Hg <superscript>2+</superscript> -activated and ICT-based NIR emission fluorescent probe NIR-HO for sequentially monitoring the ONOO <superscript>-</superscript> level with a "dual-key-and-lock" strategy. The probe NIR-HO showed rapid response and excellent specificity and sensitivity for the detection of Hg <superscript>2+</superscript> and ONOO <superscript>-</superscript> in vitro. Cell imaging demonstrated that Hg <superscript>2+</superscript> -induced oxidative stress was involved in ONOO <superscript>-</superscript> upregulation. Also, GSH, NAC, and EDTA were employed as excellent detoxifying drugs against Hg <superscript>2+</superscript> -induced toxicity. Moreover, the probe NIR-HO was successfully used for imaging Hg <superscript>2+</superscript> and ONOO <superscript>-</superscript> in vivo. In brief, NIR-HO provides a simple and powerful approach which can be used to image Hg <superscript>2+</superscript> -induced oxidative stress in the pathological environment.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 92
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32786484
- Full Text :
- https://doi.org/10.1021/acs.analchem.0c02509