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Alpha-lipoic acid ameliorates H 2 O 2 -induced human vein endothelial cells injury via suppression of inflammation and oxidative stress.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2020 Nov; Vol. 84 (11), pp. 2253-2263. Date of Electronic Publication: 2020 Aug 13. - Publication Year :
- 2020
-
Abstract
- The study was aimed to investigate the effect of alpha-lipoic acid (ALA) on human umbilical vein endothelial cells (HUVECs) injury induced by hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) and to explore its possible mechanisms. We established the H <subscript>2</subscript> O <subscript>2</subscript> -induced HUVECs injury model and the ALA treatment groups in which HUVECs were co-incubated with H <subscript>2</subscript> O <subscript>2</subscript> (250 μmol/L) and different final concentrations of ALA (100,200,400 μmol/L) for 48 h. Cell survival rate assay and LDH activity assay were carried out. The levels of related proteins were performed by Western Blot. We observed that H <subscript>2</subscript> O <subscript>2</subscript> administration resulted in an increase in the LDH activity and a decrease in cell survival rate. The expression levels of Nox4, Bax, NF-κB p65, Caspase-9, Caspase-3, iNOS, VCAM-1 and ICAM-1 were up-regulated, while the expression level of Bcl-2 was down-regulated. All these factors were significantly improved by ALA treatment. In brief, ALA treatment ameliorates H <subscript>2</subscript> O <subscript>2</subscript> -induced HUVECs damage by inhibiting inflammation and oxidative stress.
- Subjects :
- Apoptosis drug effects
Cytoprotection drug effects
Down-Regulation drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Inflammation pathology
L-Lactate Dehydrogenase metabolism
NADPH Oxidase 4 metabolism
NF-kappa B metabolism
Signal Transduction drug effects
Human Umbilical Vein Endothelial Cells cytology
Human Umbilical Vein Endothelial Cells drug effects
Hydrogen Peroxide adverse effects
Oxidative Stress drug effects
Thioctic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 84
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32787513
- Full Text :
- https://doi.org/10.1080/09168451.2020.1802221