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Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast.
- Source :
-
PLoS genetics [PLoS Genet] 2020 Aug 18; Vol. 16 (8), pp. e1008569. Date of Electronic Publication: 2020 Aug 18 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Correct bioriented attachment of sister chromatids to the mitotic spindle is essential for chromosome segregation. In budding yeast, the conserved protein shugoshin (Sgo1) contributes to biorientation by recruiting the protein phosphatase PP2A-Rts1 and the condensin complex to centromeres. Using peptide prints, we identified a Serine-Rich Motif (SRM) of Sgo1 that mediates the interaction with condensin and is essential for centromeric condensin recruitment and the establishment of biorientation. We show that the interaction is regulated via phosphorylation within the SRM and we determined the phospho-sites using mass spectrometry. Analysis of the phosphomimic and phosphoresistant mutants revealed that SRM phosphorylation disrupts the shugoshin-condensin interaction. We present evidence that Mps1, a central kinase in the spindle assembly checkpoint, directly phosphorylates Sgo1 within the SRM to regulate the interaction with condensin and thereby condensin localization to centromeres. Our findings identify novel mechanisms that control shugoshin activity at the centromere in budding yeast.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Amino Acid Motifs
Nuclear Proteins chemistry
Nuclear Proteins genetics
Phosphorylation
Protein Binding
Protein Phosphatase 2 metabolism
Protein Serine-Threonine Kinases metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins chemistry
Saccharomyces cerevisiae Proteins genetics
Adenosine Triphosphatases metabolism
Centromere metabolism
DNA-Binding Proteins metabolism
Multiprotein Complexes metabolism
Nuclear Proteins metabolism
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 16
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 32810145
- Full Text :
- https://doi.org/10.1371/journal.pgen.1008569