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CD4 and CD8 double-negative immunophenotype of thymoma-associated lymphocytes in a dog.
- Source :
-
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2020 Nov; Vol. 32 (6), pp. 918-922. Date of Electronic Publication: 2020 Aug 19. - Publication Year :
- 2020
-
Abstract
- Persistent small-cell lymphocytosis in dogs with a concurrent mediastinal mass has been associated with both thymoma and small-cell lymphoma. In thymomas, neoplastic thymic epithelial cells induce overproduction and release of polyclonal lymphocytes, whereas thymic lymphoma results in thymic effacement by a clonal expansion of neoplastic lymphocytes and subsequent leukemic phase of lymphoma. Flow cytometry has been used to differentiate these 2 entities by immunophenotyping mediastinal mass aspirates. It has been reported that cases with mediastinal masses in which ≥ 10% of the associated small-cell lymphocytes were double positive for CD4 and CD8 were thymomas, whereas masses associated with < 10% were suggestive of lymphoma. We report a unique case of thymoma-associated lymphocytosis lacking the classic CD4+CD8+ immunophenotype. Our findings suggest that there may be more diversity in the thymoma-associated lymphocyte immunophenotype than has been identified previously; immunophenotyping alone might not be sufficient to differentiate thymic small-cell lymphoma from thymoma-associated lymphocytosis. In dogs with mediastinal masses and peripheral lymphocytosis, employing a variety of testing modalities to avoid misdiagnosis is prudent. These modalities include cytologic and/or histologic evaluation, immunophenotyping, and clonality assessment.
- Subjects :
- Animals
Dogs
Female
Flow Cytometry veterinary
Lymphocytosis diagnosis
Lymphocytosis pathology
Lymphoma pathology
Lymphoma veterinary
Male
T-Lymphocytes classification
Thymoma diagnosis
Thymoma pathology
Dog Diseases diagnosis
Immunophenotyping veterinary
Lymphocytosis veterinary
T-Lymphocytes metabolism
Thymoma veterinary
Thymus Neoplasms veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1943-4936
- Volume :
- 32
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 32814519
- Full Text :
- https://doi.org/10.1177/1040638720948628