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Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro.
- Source :
-
BMC oral health [BMC Oral Health] 2020 Aug 21; Vol. 20 (1), pp. 230. Date of Electronic Publication: 2020 Aug 21. - Publication Year :
- 2020
-
Abstract
- Background: Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents.<br />Methods: To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human saliva and dental plaque. These biofilms were subject to "shooting" treatments with a commercial high velocity microspray (HVM) device.<br />Results: HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qPCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions.<br />Conclusion: We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.
- Subjects :
- Biofilms
Humans
Oxygen
Saliva
Dental Plaque
Microbiota
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6831
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC oral health
- Publication Type :
- Academic Journal
- Accession number :
- 32825831
- Full Text :
- https://doi.org/10.1186/s12903-020-01217-0