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Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS.
- Source :
-
Annals of clinical and translational neurology [Ann Clin Transl Neurol] 2020 Sep; Vol. 7 (9), pp. 1628-1641. Date of Electronic Publication: 2020 Aug 23. - Publication Year :
- 2020
-
Abstract
- Objective: To investigate the effect of cholecalciferol (vitamin D3) supplementation on peripheral immune cell frequency and N-glycan branching in patients with relapsing-remitting multiple sclerosis (RRMS).<br />Methods: Exploratory analysis of high-dose (20 400 IU) and low-dose (400 IU) vitamin D3 supplementation taken every other day of an 18-month randomized controlled clinical trial including 38 RRMS patients on stable immunomodulatory therapy (NCT01440062). We investigated cholecalciferol treatment effects on N-glycan branching using L-PHA stain (phaseolus vulgaris leukoagglutinin) at 6 months and frequencies of T-, B-, and NK-cell subpopulations at 12 months with flow cytometry.<br />Results: High-dose supplementation did not change CD3+ T cell subsets, CD19+ B cells subsets, and NK cells frequencies, except for CD8+ T regulatory cells, which were reduced in the low-dose arm compared to the high-dose arm at 12 months. High-dose supplementation decreased N-glycan branching on T and NK cells, measured as L-PHA mean fluorescence intensity (MFI). A reduction of N-glycan branching in B cells was not significant. In contrast, low-dose supplementation did not affect N-glycan branching. Changes in N-glycan branching did not correlate with cell frequencies.<br />Interpretation: Immunomodulatory effect of vitamin D may involve regulation of N-glycan branching in vivo. Vitamin D3 supplementation did at large not affect the frequencies of peripheral immune cells.<br /> (© 2020 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.)
- Subjects :
- Adult
Cholecalciferol administration & dosage
Female
Humans
Immunologic Factors administration & dosage
Male
Middle Aged
Treatment Outcome
B-Lymphocyte Subsets drug effects
Cholecalciferol pharmacology
Dietary Supplements
Immunologic Factors pharmacology
Killer Cells, Natural drug effects
Multiple Sclerosis, Relapsing-Remitting drug therapy
Polysaccharides metabolism
T-Lymphocyte Subsets drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2328-9503
- Volume :
- 7
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Annals of clinical and translational neurology
- Publication Type :
- Academic Journal
- Accession number :
- 32830462
- Full Text :
- https://doi.org/10.1002/acn3.51148