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Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.
- Source :
-
PloS one [PLoS One] 2020 Aug 25; Vol. 15 (8), pp. e0238189. Date of Electronic Publication: 2020 Aug 25 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- The use of reference genes is required for relative quantification in gene expression analysis and the stability of these genes can be variable depending on the experimental design. Therefore, it is indispensable to test the reliability of endogenous genes previously to their use. This study evaluated nine candidate reference genes to select the most stable genes to be used as reference in gene expression studies with the femoral cartilage of normal and epiphysiolysis-affected broilers. The femur articular cartilage of 29 male broilers with 35 days of age was collected, frozen and further submitted to RNA extraction and quantitative PCR (qPCR) analysis. The candidate reference genes evaluated were GAPDH, HMBS, HPRT1, MRPS27, MRPS30, RPL30, RPL4, RPL5, and RPLP1. For the gene stability evaluation, three software were used: GeNorm, BestKeeper and NormFinder, and a global ranking was generated using the function RankAggreg. In this study, the RPLP1 and RPL5 were the most reliable endogenous genes being recommended for expression studies with femur cartilage in broilers with epiphysiolysis and possible other femur anomalies.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Algorithms
Animals
Bird Diseases metabolism
Chickens metabolism
Epiphyses, Slipped genetics
Epiphyses, Slipped metabolism
Femur
Gene Expression
Gene Expression Profiling statistics & numerical data
Gene Expression Profiling veterinary
Male
RNA, Messenger genetics
RNA, Messenger metabolism
Real-Time Polymerase Chain Reaction
Bird Diseases genetics
Cartilage, Articular metabolism
Chickens genetics
Epiphyses, Slipped veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 15
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 32841273
- Full Text :
- https://doi.org/10.1371/journal.pone.0238189