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Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia.
- Source :
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Systematic and applied microbiology [Syst Appl Microbiol] 2020 Sep; Vol. 43 (5), pp. 126126. Date of Electronic Publication: 2020 Aug 05. - Publication Year :
- 2020
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Abstract
- A novel anaerobic moderately thermophilic bacterium, designated strain 38H-str <superscript>T</superscript> , was isolated from a 12m deep hot spring of the Kunashir Island shore. Gram-negative cells were non-spore-forming, motile, straight or curved filamentous rods, occasionally forming loops and knots. The strain grew at 20-65°C and pH range of 4.0-9.0 with an optimum at 50°C and pH 6.5-7.0. Strain 38H-str <superscript>T</superscript> required 0.5-2.5% NaCl (1.5% is an optimum) for growth. It was a chemoorganoheterotroph, growing on carbohydrates (starch, pullulan, alginate, laminarin, beta-glucan) or peptide mixtures and proteins (peptone, tryptone, gelatin, and α- or β- keratins). Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso- and anteiso-C <subscript>15:0</subscript> . Phosphatidylethanolamine, an unidentified phospholipid, and three unidentified polar lipids were detected in cellular lipids fractions. The quinone was MK-7. The size of complete genome of strain 38H-str <superscript>T</superscript> was 3.2 Mb; DNA G+C content was 38.3mol%. According to 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 38H-str <superscript>T</superscript> represented a deeply branched lineage near the root of the class Bacteroidia. Based on phylogenetic analysis and phenotypic features the novel isolate was assigned to a novel family within the order Bacteroidales for which the name Tenuifilaceae fam. nov. is proposed. Strain 38H-str <superscript>T</superscript> (=DSM 100343 <superscript>T</superscript> =VKM B-2964 <superscript>T</superscript> ) represents the first genus and species Tenuifilum thalassicum gen. nov., sp. nov.<br />Competing Interests: Declaration of Competing Interest The authors report no declarations of interest.<br /> (Copyright © 2020 Elsevier GmbH. All rights reserved.)
- Subjects :
- Anaerobiosis
Bacteroidetes genetics
Bacteroidetes physiology
Base Composition
Carbohydrate Metabolism
Carbohydrates
DNA, Bacterial chemistry
DNA, Bacterial genetics
Fatty Acids analysis
Fermentation
Genes, Bacterial
Genes, rRNA
Genome, Bacterial
Hot Temperature
Lipids analysis
Peptides metabolism
Phenotype
Phylogeny
Proteins metabolism
RNA, Ribosomal, 16S genetics
Russia
Sequence Analysis, DNA
Bacteroidetes classification
Bacteroidetes isolation & purification
Hot Springs microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0984
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Systematic and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32847792
- Full Text :
- https://doi.org/10.1016/j.syapm.2020.126126