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Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies.
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies.
- Source :
-
Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2020 Jul; Vol. 21, pp. 100438. Date of Electronic Publication: 2020 Jul 22. - Publication Year :
- 2020
-
Abstract
- Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antibodies, Protozoan blood
Argentina
Cattle
Cattle Diseases parasitology
Coccidiosis diagnosis
Coccidiosis parasitology
Female
Pregnancy
Pregnancy Complications, Parasitic diagnosis
Pregnancy Complications, Parasitic parasitology
Cattle Diseases diagnosis
Coccidiosis veterinary
Fetus parasitology
Neospora isolation & purification
Pregnancy Complications, Parasitic veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 2405-9390
- Volume :
- 21
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology, regional studies and reports
- Publication Type :
- Academic Journal
- Accession number :
- 32862902
- Full Text :
- https://doi.org/10.1016/j.vprsr.2020.100438