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The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2020 Dec 01; Vol. 518, pp. 111022. Date of Electronic Publication: 2020 Aug 29. - Publication Year :
- 2020
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Abstract
- Blood glucose is of great importance to development and metabolic homeostasis in fetuses. Stimulation of harmful factors during gestation induces pathoglycemia. Angiotensin II type 1 receptor autoantibody (AT1-AA), a newly discovered gestational harmful factor, has been shown to induce intrauterine growth restriction in fetuses and glucose disorders in adults. However, whether and how AT1-AA influences the blood glucose level of fetuses during gestation is not yet clear. The purpose of the current study was to observe the fetal blood glucose level of AT1-AA-positive pregnant rats during late pregnancy and to determine the roles that hepatic glucose transporters play in this process. We established AT1-AA-positive pregnant rats by injecting AT1-AA into the caudal veins of rats in the 2nd trimester of gestation. Although the fetal blood glucose level in the 3rd trimester of gestation decreased, hepatic glucose uptake increased detected. Through separating membrane and cytosolic proteins, we demonstrated that both the expression and membrane transport ratio of glucose transporter 1 (GLUT1), which is responsible for glucose transport in fetal hepatocytes, were upregulated, accompanied by increased expression of N-glycosyltransferase STT3A, which contributes to the N-glycosylation of GLUT1. In vitro, we identified that AT1-AA increased glucose uptake, the expression and membrane transport ratio of GLUT1 and the expression of STT3A in HepG2 cell lines via separating membrane and cytosolic proteins and immunofluorescence, resulting in the decreased glucose content in the medium. The GLUT1 inhibitor WZB117 reversed the decreases in glucose content in the medium, the increases in glucose uptake, the increases in the expression and membrane transport ratio of GLUT1 caused by AT1-AA. The N-glycosyltransferase inhibitor NGI as well as si-STT3A reversed the AT1-AA-induced upregulation of the STT3A-GLUT1-glucose uptake effect. This study demonstrates that AT1-AA lowers the blood glucose level of fetuses via the STT3A-GLUT1-glucose uptake axis in liver.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Autoantibodies adverse effects
Embryo, Mammalian
Female
Fetus immunology
Fetus metabolism
Glucose Transporter Type 1 metabolism
Hep G2 Cells
Hexosyltransferases metabolism
Humans
Hypoglycemia immunology
Hypoglycemia metabolism
Liver immunology
Male
Membrane Proteins metabolism
Pregnancy
Rats
Rats, Sprague-Dawley
Signal Transduction
Autoantibodies physiology
Glucose metabolism
Hypoglycemia etiology
Liver metabolism
Receptor, Angiotensin, Type 1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8057
- Volume :
- 518
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 32871226
- Full Text :
- https://doi.org/10.1016/j.mce.2020.111022