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First molecular detection of Pneumocystis spp. in red foxes (Vulpes vulpeslinnaeus, 1758) and raccoon dogs (Nyctereutes procyonoidesgray, 1834).
- Source :
-
Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2020 Dec; Vol. 73, pp. 101531. Date of Electronic Publication: 2020 Aug 14. - Publication Year :
- 2020
-
Abstract
- Fungal organisms of the genus Pneumocystis may cause Pneumocystis pneumonia (PCP) in humans, but also domestic and wild mammals. Almost every animal species hosts its own genetically distinct Pneumocystis species, however information is sparse. In this study, 62 red foxes (Vulpes vulpes) and 37 raccoon dogs (Nyctereutes procyonoides) were collected in North-East Germany. The lung tissues of the animals were analysed by a new designed specific pan-Pneumocystis mtLSU rRNA gene PCR and sequencing. With this PCR, detection and discrimination of all known Pneumocystis spp. in a single step should be possible. This first detection of Pneumocystis spp. in 29/62 (46.8%) red foxes and 29/37 (78.4%) raccoon dogs indicated, that they harbour two dissimilar strains, as seen by specific single nucleotide position changes (SNPs). Nevertheless, five samples with contrary SNPs showed a probable inter-species transmission.<br /> (Copyright © 2020. Published by Elsevier Ltd.)
- Subjects :
- Animals
DNA, Fungal isolation & purification
Female
Lung microbiology
Male
Phylogeny
Pneumocystis classification
Pneumocystis genetics
Pneumonia, Pneumocystis diagnosis
Pneumonia, Pneumocystis epidemiology
Polymerase Chain Reaction veterinary
Retrospective Studies
Foxes
Pneumocystis isolation & purification
Pneumonia, Pneumocystis veterinary
Raccoon Dogs
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1667
- Volume :
- 73
- Database :
- MEDLINE
- Journal :
- Comparative immunology, microbiology and infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 32871298
- Full Text :
- https://doi.org/10.1016/j.cimid.2020.101531