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First molecular detection of Pneumocystis spp. in red foxes (Vulpes vulpeslinnaeus, 1758) and raccoon dogs (Nyctereutes procyonoidesgray, 1834).

Authors :
Riebold D
Lubig J
Wolf P
Wolf C
Russow K
Loebermann M
Slevogt H
Mohr E
Feldhusen F
Reisinger EC
Source :
Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2020 Dec; Vol. 73, pp. 101531. Date of Electronic Publication: 2020 Aug 14.
Publication Year :
2020

Abstract

Fungal organisms of the genus Pneumocystis may cause Pneumocystis pneumonia (PCP) in humans, but also domestic and wild mammals. Almost every animal species hosts its own genetically distinct Pneumocystis species, however information is sparse. In this study, 62 red foxes (Vulpes vulpes) and 37 raccoon dogs (Nyctereutes procyonoides) were collected in North-East Germany. The lung tissues of the animals were analysed by a new designed specific pan-Pneumocystis mtLSU rRNA gene PCR and sequencing. With this PCR, detection and discrimination of all known Pneumocystis spp. in a single step should be possible. This first detection of Pneumocystis spp. in 29/62 (46.8%) red foxes and 29/37 (78.4%) raccoon dogs indicated, that they harbour two dissimilar strains, as seen by specific single nucleotide position changes (SNPs). Nevertheless, five samples with contrary SNPs showed a probable inter-species transmission.<br /> (Copyright © 2020. Published by Elsevier Ltd.)

Details

Language :
English
ISSN :
1878-1667
Volume :
73
Database :
MEDLINE
Journal :
Comparative immunology, microbiology and infectious diseases
Publication Type :
Academic Journal
Accession number :
32871298
Full Text :
https://doi.org/10.1016/j.cimid.2020.101531