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Helicobacter genus in the intestine and liver of stray cats: the molecular, histopathological, and immunohistochemical study.
- Source :
-
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2020 Dec; Vol. 51 (4), pp. 2123-2132. Date of Electronic Publication: 2020 Sep 01. - Publication Year :
- 2020
-
Abstract
- Objectives: The study was designed to determine the presence of Helicobacter genus and three species of H. pylori, H. bilis, and H. canis, in the duodenum, ileum, colon, and liver of stray cats. Moreover, the histopathological and immunohistochemical analyses have been performed.<br />Methods: Samples were taken from the duodenum, ileum, colon, and liver of 30 cats for molecular and histopathological evaluations. Polymerase chain reaction was carried out for the detection of the Helicobacter genus in the mentioned samples. Then, species-specific primers were used in Helicobacter-positive samples.<br />Results: Helicobacter genus prevalence rates in the duodenum, ileum, colon, and liver samples were 50%, 60%, 50%, and 43.3%, respectively. Helicobacter pylori, H. canis, and H. bilis were isolated from at least one tissue of 18 (60%), 13 (43.3%), and 8 (26.7%) of the cats, respectively. Immunohistochemical findings confirmed the presence of bacteria in the intestinal crypt or the mucosal layer of duodenum, ileum, colon, and hepatic sinusoids.<br />Conclusion: In the present study, the concurrent infection of duodenum and liver was noticeable. Furthermore, the high prevalence of H. pylori in cats, as a well-known human pathogen, should be considered. High incidence of Helicobacter in gut and liver of Ahvaz stray cats is noticeable. According to the zoonotic importance of Helicobacter, more studies in the field of treatment and prevention are highly recommended.
- Subjects :
- Animals
Cats microbiology
DNA, Bacterial genetics
Helicobacter isolation & purification
Helicobacter Infections epidemiology
Helicobacter Infections pathology
Immunohistochemistry
Iran epidemiology
Prevalence
RNA, Ribosomal, 16S genetics
Animals, Wild microbiology
Helicobacter classification
Helicobacter Infections veterinary
Intestines microbiology
Liver microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1678-4405
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Publication Type :
- Academic Journal
- Accession number :
- 32875544
- Full Text :
- https://doi.org/10.1007/s42770-020-00359-1